Abstract:
Fasciola gigantica diagnosis usually performed by detection of worm eggs presence in the feces,
but this conventional method has many disadvantages. Early diagnosis (early detection) cannot be
performed in conventional methods because the worms in the host's body began to lay eggs at the age
of 8–12 weeks of patency. The current detection method that is based on antibodyantigen
reactions using
excreted/secreted (ES) liquid by adult F. gigantica, is believed to be used for the early detection of
fasciolosis. This study aimed to characterize the antigenic components of F. gigantica extretory/secretory
products that could be used as a vaccine candidate development for early fasciolosis diagnostics. ES
products were separated by PEG4000 at various concentrations (8%, 16%, 24%), then precipitates (pellets)
obtained were dialyzed and characterized using SDSPAGE
and Western blotting. Results from SDSPAGE
showed that there were 18 proteins bands with 7–70 kDa molecular weights. Western blotting on
pellets derived from PEG separation at various concentrations affirmed that the proteins of 50, 25 and
20 kDa were antigenic at 8% PEG concentration, the 25 kDa and 50 kDa were antigenic at 16% PEG
concentrations and the 25 kDa was antigenic at 25% PEG concentration
