The use biological agent Pseudomonas fluorescens P60 of seed health, physiological quality, and growth of Seedling IR 64

Abstract

The purpose of the research were: (1) test the IR 64 seed health, (2) test P. flourescens P60 in improving seed health, (3) test P. Fluorescents P60 on physiological seed quality, (4) test P. flourescens P60 on the growth of seedlings in a greenhouse. The results showed IR-64 infected seed borne pathogens of Aspergillus flavus, Alternaria padwickii, Pseudomonas glumae and Pseudomonas syringae. Testing of P. flourescens P60 in improving seed health, indicating P. flourescens P60 could improve seed health by inhibiting the growth of the colony A. padwickii, A. flavus, and P. glumae, but it did not inhibit the growth of colonies of P. Syringae. While testing P. flourescens P60 to physiological quality of seeds, P. flourescens P60 was able to maintain the physiological quality with germination above 80%. The results in the greenhouse showed that P. flourescens P60 with soaking time 15 minutes and 25 minutes had the same effect on seedling height, root length and seedling dry weight. However, 25 minutes soaking time resulted in heavy wet seed and root dry weight were higher than soaking time of 15 minutes