Objective: Effort to develop an environmental-friendly oriented method of animal leather tanning, especially de-hairing process, becomes a concern of this research. The aim was to purify the alkaline protease from Bacillus ...

Alkaline protease enzyme from Bacillus cereus LS2B was successively purified by three steps procedure including ammonium precipitation, membrane dialysis, and HiTrap ion exchange chromatography with DEAE Sepharose FF ...